| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096162 |
| Chromosome: | chromosome 8 |
| Location: | 62466 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358522 | CEP5 | Cysteine endopeptidase; (1 of 1) PTHR12411//PTHR12411:SF372 - CYSTEINE PROTEASE FAMILY C1-RELATED // PROTEIN TAG-329 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGGACAGCTCAGCCAGGACTGGCCAGG |
| Internal bar code: | CTAACTTGGGAACACGGCAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 95 |
| LEAP-Seq percent confirming: | 99.5496 |
| LEAP-Seq n confirming: | 221 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAGTCGGAGCTGTCCCAG |
| Suggested primer 2: | AGTCAATCTCCCCTCGTCCT |