| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.096183 |
| Chromosome: | chromosome 10 |
| Location: | 4286579 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450650 | (1 of 34) PF00168 - C2 domain (C2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTACAAGGCCATAGCGCGCACACAGGTG |
| Internal bar code: | CCCCTGTTGTTCATCTGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 209 |
| LEAP-Seq percent confirming: | 98.7013 |
| LEAP-Seq n confirming: | 228 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGACTTACACCAGGCTT |
| Suggested primer 2: | TAAGGTGCTCATTGTCGCTG |