Insertion junction: LMJ.RY0402.096192_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.096192
Chromosome:chromosome 4
Location:2693502
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g223300 CCP1 (1 of 5) K15109 - solute carrier family 25 (mitochondrial carnitine/acylcarnitine transporter), member 20/29 (SLC25A20_29, CACT, CACL, CRC1); Low-CO2-inducible chloroplast envelope protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTTAAATGATGATTTTGACTGCTGTTTT
Internal bar code:AAGGTTTACCTCGTCGGGTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:135
LEAP-Seq percent confirming:69.3694
LEAP-Seq n confirming:539
LEAP-Seq n nonconfirming:238
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTACGAGGAGAACGTGGAGC
Suggested primer 2:GTGGTCCTGAGAGGGTGTGT