| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.096229 |
| Chromosome: | chromosome 8 |
| Location: | 3812492 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377450 | (1 of 1) PTHR10997:SF9 - IMPORTIN-9 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCATGTCTGTCCTGAAAGCGTAAGGGCAG |
| Internal bar code: | CGCGCGCGTCCACACTTAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 98.6857 |
| LEAP-Seq n confirming: | 3379 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGCGAACTAGACGAGGC |
| Suggested primer 2: | CTTGGGTAATCTCCGAGCTG |