| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.096253 |
| Chromosome: | chromosome 2 |
| Location: | 5153069 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105350 | (1 of 2) PF03619 - Organic solute transporter Ostalpha (Solute_trans_a) | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGGCCCGCGGCCGGGCGCGGCCTGGCG |
| Internal bar code: | ACGGTCTACAGGCCCCGCGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 99.3827 |
| LEAP-Seq n confirming: | 161 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGCCATGAGATAGGCGA |
| Suggested primer 2: | CTGGGAGTTTCAAAAGCAGC |