Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.096268 |
Chromosome: | chromosome 1 |
Location: | 104046 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g000750 | ZFR2 | zinc finger protein; (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAACTGTCAACACAACTCGACCCTCGCC |
Internal bar code: | ACGACTAGCGGAGACTGGGGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 626 |
LEAP-Seq percent confirming: | 98.7448 |
LEAP-Seq n confirming: | 708 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTCTACGGGTTTCCTGCCT |
Suggested primer 2: | CTGCTGCGTCATCTGCTATG |