Insertion junction: LMJ.RY0402.096268_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.096268
Chromosome:chromosome 1
Location:104046
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g000750 ZFR2 zinc finger protein; (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAAACTGTCAACACAACTCGACCCTCGCC
Internal bar code:ACGACTAGCGGAGACTGGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:626
LEAP-Seq percent confirming:98.7448
LEAP-Seq n confirming:708
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCTACGGGTTTCCTGCCT
Suggested primer 2:CTGCTGCGTCATCTGCTATG