Insertion junction: LMJ.RY0402.096287_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.096287
Chromosome:scaffold 19
Location:98531
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre19.g750647 (1 of 1) PF01636//PF07714 - Phosphotransferase enzyme family (APH) // Protein tyrosine kinase (Pkinase_Tyr) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTGAGACTGCGGTGGTGAGAGGTGGGGT
Internal bar code:AAAAGGGGCGGACGTCAGGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:27
LEAP-Seq percent confirming:30.11
LEAP-Seq n confirming:1068
LEAP-Seq n nonconfirming:2479
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GACCGCAGAGTTTTAGTCGC
Suggested primer 2:GGGTGTAGGTGCGTGTAGGT