| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.096313 |
| Chromosome: | chromosome 13 |
| Location: | 2388878 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579550 | CGL27 | Conserved in the Green Lineage; (1 of 5) IPR002645 - STAS domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCATTTACAGCAGGCTACCAGCCTTCATG |
| Internal bar code: | TACATACATAAAGTTTAAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 99.8314 |
| LEAP-Seq n confirming: | 592 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTGGACAGGGAGGTTTG |
| Suggested primer 2: | TAGGAAGAAGAGGGGGAGGA |