| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.096337 |
| Chromosome: | chromosome 16 |
| Location: | 3505724 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675958 | (1 of 2) PTHR10766//PTHR10766:SF49 - TRANSMEMBRANE 9 SUPERFAMILY PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGGGCAGTTTTTCCACGTACAGCGGTG |
| Internal bar code: | CTTTACTCAGAAATTCGATGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 98.2882 |
| LEAP-Seq n confirming: | 689 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGCCTGCACTACAAAAT |
| Suggested primer 2: | AAACCTGGTGAAACTGCCAC |