| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096345 |
| Chromosome: | chromosome 16 |
| Location: | 1934847 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656200 | (1 of 1) PF00612//PF13424 - IQ calmodulin-binding motif (IQ) // Tetratricopeptide repeat (TPR_12) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGACGAAGTGCTGGAGTTGCACGCCGAC |
| Internal bar code: | CGCCGGTTGAGGTTACGCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 97.4578 |
| LEAP-Seq n confirming: | 3872 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGTCGCAACTGAGCGAG |
| Suggested primer 2: | GTCTGTCAGCTCCGTCATCA |