| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.096349 |
| Chromosome: | chromosome 11 |
| Location: | 3535224 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g481400 | (1 of 1) K08864 - tousled-like kinase [EC:2.7.11.1] (TLK) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGCGGGAAGTATCCTACTAGGGTCAGG |
| Internal bar code: | GAAAGTTGAATGGGGAGCCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 54.5882 |
| LEAP-Seq n confirming: | 1511 |
| LEAP-Seq n nonconfirming: | 1257 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGCGTCGACCCACTAAT |
| Suggested primer 2: | GGCAGGATGTAAGTAGGCCA |