| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096350 |
| Chromosome: | chromosome 3 |
| Location: | 8561254 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202113 | (1 of 1) 6.3.2.6 - Phosphoribosylaminoimidazolesuccinocarboxamide synthase / SAICARs | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCGGTCCATGCGTGGATGCGAGCGGAG |
| Internal bar code: | CGGGGTTAGACAAAAAGGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 69.1824 |
| LEAP-Seq n confirming: | 110 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGCAGAGCACAGAAGGG |
| Suggested primer 2: | GAGGGGGTAAGGGTCAAGAG |