| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.096370 |
| Chromosome: | chromosome 9 |
| Location: | 3473282 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389504 | (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGCGGTCCAGCTGTCATGCCACGTAAGT |
| Internal bar code: | TAGGTCGTCTGGGCGTGTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 214 |
| LEAP-Seq percent confirming: | 68.7793 |
| LEAP-Seq n confirming: | 1172 |
| LEAP-Seq n nonconfirming: | 532 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGAGCATTGGGAGGTGTG |
| Suggested primer 2: | GCACATTGTCCATTCGTCAC |