Insertion junction: LMJ.RY0402.096380_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.096380
Chromosome:chromosome 9
Location:6599705
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g408428 UMAMIT (Usually Multiple Acids Move In and Out Transporters) type transporter; (1 of 3) PTHR11132//PTHR11132:SF125 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAGGCTGCAAAGCCCACCATACTAATGC
Internal bar code:CGGTTGTGGGGGCATTTCGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:574
LEAP-Seq percent confirming:94.6899
LEAP-Seq n confirming:9023
LEAP-Seq n nonconfirming:506
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTTCGGCGGCAATATCTA
Suggested primer 2:CACGTGAAGCCCACAGAGTA