| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096381 |
| Chromosome: | chromosome 2 |
| Location: | 7082824 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g147050 | (1 of 7) PF05517 - p25-alpha (p25-alpha); P25 Alpha Domain Containing Flagellar Associated Protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAAGGTAGGAGCGCGACTGGCGGGAGAG |
| Internal bar code: | ACCAGAGTGGGTAACAGGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 641 |
| LEAP-Seq percent confirming: | 99.1798 |
| LEAP-Seq n confirming: | 10399 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACCTGAATGTATGTTGC |
| Suggested primer 2: | TATTCCTCGGCCTATGTTGC |