| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.096385 |
| Chromosome: | chromosome 2 |
| Location: | 6865499 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119550 | NIP1 | NaCl-inducible protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCCCTAGCTATGACAGCAGCAGGCGTG |
| Internal bar code: | GAATGTGTGGCCATGATTGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 133 |
| LEAP-Seq percent confirming: | 43.3773 |
| LEAP-Seq n confirming: | 822 |
| LEAP-Seq n nonconfirming: | 1073 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGAATGGTAGGGGATTAT |
| Suggested primer 2: | GGAAGCACGTGCACTACTGA |