Insertion junction: LMJ.RY0402.096388_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.096388
Chromosome:chromosome 9
Location:6995706
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g410900 NAR2,NIT8 nitrite transporter accessory protein; (1 of 1) PF16974 - High-affinity nitrate transporter accessory (NAR2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGCTTTGCGACCACCATGCGCAGGGGAT
Internal bar code:TGACGGGATCGGCACTCAAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:527
LEAP-Seq percent confirming:99.2926
LEAP-Seq n confirming:2667
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAGGTTTAGGGACAGGTG
Suggested primer 2:TGCTTTCGATGAGTGCAAAC