| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096397 |
| Chromosome: | chromosome 12 |
| Location: | 1520602 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487100 | TSM2 | Methionyl-tRNA synthetase; (1 of 1) PTHR11946//PTHR11946:SF89 - ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHIONYL-TRNA SYNTHETASES // METHIONYL-TRNA SYNTHETASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGACCTGCCACTTCGCCAGCTGGCGGC |
| Internal bar code: | CATTGCCCTAGGTTGGGCACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 68 |
| LEAP-Seq percent confirming: | 98.1308 |
| LEAP-Seq n confirming: | 105 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACATAACCCCTTCCCTTT |
| Suggested primer 2: | TCCCTTATACGCACAGACCC |