| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.096408 |
| Chromosome: | chromosome 7 |
| Location: | 1957442 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325751 | FAP258 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; Flagellar Associated Protein 258 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGAGGGTACTGCCTAGGTACCAACTTGT |
| Internal bar code: | AGTTTAGCCGATTTTACTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 354 |
| LEAP-Seq percent confirming: | 94.6927 |
| LEAP-Seq n confirming: | 1017 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGATGACTACGCGAATG |
| Suggested primer 2: | AGAAGCTACGGGAGAGGAGG |