| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096415 |
| Chromosome: | chromosome 2 |
| Location: | 4264986 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098950 | SYP72 | Qc-SNARE protein, SYP7-family; (1 of 2) K08506 - syntaxin of plants SYP7 (SYP7) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCAGCGCGCGCATTTGCATAGGGAAGG |
| Internal bar code: | GGAAGTGATTATTTCTAATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 89.7783 |
| LEAP-Seq n confirming: | 729 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTACTCCACCCGAGTCCT |
| Suggested primer 2: | GGTCGTAGATGGACATGGCT |