| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.096435 |
| Chromosome: | chromosome 5 |
| Location: | 3127666 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239200 | FAP275 | Flagellar Associated Protein 275 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGCGGGCCCGCGGTAACCCGGCGACGG |
| Internal bar code: | TGCTGGGCTTTCTTGTCGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1021 |
| LEAP-Seq percent confirming: | 99.6644 |
| LEAP-Seq n confirming: | 891 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTGAGGCAAGTCGAAAG |
| Suggested primer 2: | GATGCATGTGTCTCCCTCCT |