| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096446 |
| Chromosome: | chromosome 3 |
| Location: | 6332892 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193576 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTTGTTTGCAGTGCTGGATTGAGCGGG |
| Internal bar code: | GGACGGTTAGTTGCCACAACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 94.9086 |
| LEAP-Seq n confirming: | 14279 |
| LEAP-Seq n nonconfirming: | 766 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAATTCTTCAAGCGCACA |
| Suggested primer 2: | TGCTTGTGGGTTCACAACAT |