| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.096459 |
| Chromosome: | chromosome 12 |
| Location: | 5309701 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529050 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAAGCAACACAGCTACTGAATAGCTCAC |
| Internal bar code: | GTGGATTACCGTTGGCGGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 184 |
| LEAP-Seq percent confirming: | 47.4013 |
| LEAP-Seq n confirming: | 1669 |
| LEAP-Seq n nonconfirming: | 1852 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGACAGGGCTTCTAGGTG |
| Suggested primer 2: | ATCGCTGACTGAGCTGGATT |