Insertion junction: LMJ.RY0402.096481_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.096481
Chromosome:chromosome 2
Location:1457144
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g083750 ROC75 (1 of 1) IPR006447//IPR009057//IPR017930 - Myb domain, plants // Homeodomain-like // Myb domain; Rhythm Of Chloroplast 75 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACACCAATGTTTAGTCTGCAACCAAATG
Internal bar code:CTAAGTTCTCTATGTCCACATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1226
LEAP-Seq percent confirming:99.3304
LEAP-Seq n confirming:890
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTATTTCGAGGGGCAAGATG
Suggested primer 2:CCTTGAGCATCCCAATCAAT