| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.096555 |
| Chromosome: | chromosome 4 |
| Location: | 348161 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217910 | (1 of 25) PF00581 - Rhodanese-like domain (Rhodanese) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGCCGGGTCAAAGCGGAAGCCGACCTG |
| Internal bar code: | CTCCCGCAGCTCGAGGCGCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 82.2171 |
| LEAP-Seq n confirming: | 712 |
| LEAP-Seq n nonconfirming: | 154 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCAGGAAGGCAGGAGTA |
| Suggested primer 2: | GCTGAGGTCAACTCGGCTAC |