| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096555 |
| Chromosome: | chromosome_14 |
| Location: | 2903660 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g627500 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCACGTGCCGCATCCTGGACGAGCAACAGG |
| Internal bar code: | GGGCTCTCGGGTCGGTCACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 51 |
| LEAP-Seq percent confirming: | 74.3034 |
| LEAP-Seq n confirming: | 240 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCTGCTCATTTCACTCC |
| Suggested primer 2: | ACAGACGATGATCAGACCCC |