| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.096581 |
| Chromosome: | chromosome 14 |
| Location: | 811614 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613100 | (1 of 1) IPR008999 - Actin cross-linking | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGTAGTTGTTGGCTGGGCAGCTGGTGC |
| Internal bar code: | CGCTCAGCCTTCTTTGGATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 260 |
| LEAP-Seq percent confirming: | 99.7037 |
| LEAP-Seq n confirming: | 3365 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGTTGCTACCATGCAA |
| Suggested primer 2: | CGATGAGGAAAAAGGATGGA |