| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.096640 |
| Chromosome: | chromosome 5 |
| Location: | 3072521 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238950 | CGLD33 | (1 of 5) 2.1.1.34 - tRNA (guanosine(18)-2'-O)-methyltransferase / tRNA (Gm18) methyltransferase; Conserved in the Green Lineage and Diatoms | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCTCCAACATGGGGATCCACGCCTGCC |
| Internal bar code: | GTGTGCAGGGGGAAGCAATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 366 |
| LEAP-Seq percent confirming: | 96.3578 |
| LEAP-Seq n confirming: | 17461 |
| LEAP-Seq n nonconfirming: | 660 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTTGACTTCACAGAAGC |
| Suggested primer 2: | CACACACACACACACACGGT |