| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.096642 |
| Chromosome: | chromosome 2 |
| Location: | 5619784 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109250 | (1 of 1) PF13394 - 4Fe-4S single cluster domain (Fer4_14) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGCCAGCAGCACATACTCGATCACCAC |
| Internal bar code: | ACTTGCGACAAGCAGTACGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 467 |
| LEAP-Seq percent confirming: | 99.3609 |
| LEAP-Seq n confirming: | 7774 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGGGAGAAGGGTGGAAC |
| Suggested primer 2: | ATATGACAGACTCCCACGGC |