| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.096657 |
| Chromosome: | chromosome 12 |
| Location: | 4017557 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517000 | MAPKKK7 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; Mitogen-Activated Protein Kinase Kinase Kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGAATCCAGGCATGCCTTACTCACGGG |
| Internal bar code: | GCCCAGTGTCCGACGTGTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 70 |
| LEAP-Seq percent confirming: | 99.1254 |
| LEAP-Seq n confirming: | 340 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCCGTCTTTCAAGAACC |
| Suggested primer 2: | CACTGAGCCTTCCACACTGA |