| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096663 |
| Chromosome: | chromosome 7 |
| Location: | 3899531 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339100 | (1 of 4) PTHR24361//PTHR24361:SF411 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTGCTTCGAAGGCCGGCACTCGCTCCTC |
| Internal bar code: | ATGAGCGTACTTTTCACTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 374 |
| LEAP-Seq percent confirming: | 88.6222 |
| LEAP-Seq n confirming: | 997 |
| LEAP-Seq n nonconfirming: | 128 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGACGTGGATGTCGATG |
| Suggested primer 2: | TGTCATCCTCCTCATCCTCC |