| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096679 |
| Chromosome: | chromosome 12 |
| Location: | 8887106 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544550 | (1 of 38) IPR000719//IPR002290//IPR011009 - Protein kinase domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCCGCGGGCCCAGGCCAAGGCCCGTCC |
| Internal bar code: | TCGGGTCAGAGGGTATTGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 95.0512 |
| LEAP-Seq n confirming: | 1114 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGACTAACATGGCGGTTG |
| Suggested primer 2: | GCCTCTTCCTCTCCTCCACT |