| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.096682 |
| Chromosome: | chromosome 14 |
| Location: | 3712722 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632000 | CGE2,OPR65 | GrpE nucleotide release factor; (1 of 3) K03687 - molecular chaperone GrpE (GRPE) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCGCCGCTGCACGCTAATCGTTCTTCT |
| Internal bar code: | GCGGATTAGACGCGCGATAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 77.242 |
| LEAP-Seq n confirming: | 913 |
| LEAP-Seq n nonconfirming: | 269 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACTTTGACAACGCCAAGT |
| Suggested primer 2: | TAGAGCGCTGCTGCAATAGA |