| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096696 |
| Chromosome: | chromosome_9 |
| Location: | 6863033 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g410050 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTAAGCTTCATAAATGCATGTGAGGGCAGG |
| Internal bar code: | TCGGCCAGCTTGGGAACGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 67.1416 |
| LEAP-Seq n confirming: | 7021 |
| LEAP-Seq n nonconfirming: | 3436 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTCCTCTCCAGCACACA |
| Suggested primer 2: | CCACCTTTGTCGTGTACGTG |