| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.096713 |
| Chromosome: | chromosome 6 |
| Location: | 3715322 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278146 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATCATGCAACCCACATTCTTTCAACAGG |
| Internal bar code: | CTAAATATAGTTTTGTGGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 400 |
| LEAP-Seq percent confirming: | 97.9842 |
| LEAP-Seq n confirming: | 1361 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTCAAGACCGCAGACAG |
| Suggested primer 2: | CCTCGAACTTTTCAAGCAGG |