| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.096719 |
| Chromosome: | chromosome 4 |
| Location: | 1995115 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218750 | THB4 | Truncated hemoglobin; (1 of 1) IPR012292 - Globin/Protoglobin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGGACACTTAACGCAACATGCATGATTG |
| Internal bar code: | ATCTGATCGGGACACGCACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 90.4884 |
| LEAP-Seq n confirming: | 8467 |
| LEAP-Seq n nonconfirming: | 890 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAAATGGCCCACAACACT |
| Suggested primer 2: | AGCGACTCCGAATATGCACT |