| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096721 |
| Chromosome: | chromosome_12 |
| Location: | 6955245 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g560350 | CNK2 | NimA-related protein kinase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTTATGAGCACGTCTGATCGGAACCTGAA |
| Internal bar code: | GCGGACAGAAGGTCACACGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 710 |
| LEAP-Seq percent confirming: | 95.1006 |
| LEAP-Seq n confirming: | 4018 |
| LEAP-Seq n nonconfirming: | 207 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACAGCAACAACAGCAGC |
| Suggested primer 2: | ACCTTCTGTGGTGTCCCAAG |