| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096728 |
| Chromosome: | chromosome 2 |
| Location: | 7697902 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143050 | RPP2 | Acidic ribosomal protein P2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGAAGTGGAGTAACCATACCTCAACGCG |
| Internal bar code: | GGCGCGAAACAGCAGTTTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 500 |
| LEAP-Seq percent confirming: | 95.9158 |
| LEAP-Seq n confirming: | 775 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTTGTTCACCTTCTCGG |
| Suggested primer 2: | TTCTGCTATGCTGGTCATCG |