| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096731 |
| Chromosome: | chromosome 10 |
| Location: | 629844 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421950 | TEF26 | (1 of 4) PF12576 - Protein of unknown function (DUF3754) (DUF3754); Predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCGCGGGGCCGACAGAGAGAGCCGTGT |
| Internal bar code: | GGCCCGCAGCTCGCGCCTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 546 |
| LEAP-Seq percent confirming: | 99.594 |
| LEAP-Seq n confirming: | 736 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTCCTTGTGCACCCAAAC |
| Suggested primer 2: | ACAGGCCAAGAACGACAAGT |