| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.096773 |
| Chromosome: | chromosome 6 |
| Location: | 3730429 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278149 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCCTGTCCGTGCCTGCCTGGTGCCTG |
| Internal bar code: | GGATGGCCCTGGGTGAGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 380 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGGTGATGTGGGTTGAT |
| Suggested primer 2: | CACAGGTCGCTAACCAGACA |