Insertion junction: LMJ.RY0402.096778_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.096778
Chromosome:chromosome 3
Location:8504872
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g200879 CSR10 Carbohydrate sulfotransferase-related 10; (1 of 1) IPR000104//IPR000863//IPR012884//IPR027417 - Antifreeze protein, type I // Sulfotransferase domain // Excisionase-like // P-loop containing nucleoside triphosphate hydrolase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATCGGGCGTATATGCTGCGCGAGGGATCA
Internal bar code:ATTGGGTTGCAACAGGGGGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:65
LEAP-Seq percent confirming:53.3835
LEAP-Seq n confirming:213
LEAP-Seq n nonconfirming:186
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TAGATCATCGGAATGTGCCA
Suggested primer 2:CTAATTCGCAGCGTTTAGGC