Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.096778 |
Chromosome: | chromosome 3 |
Location: | 8504872 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g200879 | CSR10 | Carbohydrate sulfotransferase-related 10; (1 of 1) IPR000104//IPR000863//IPR012884//IPR027417 - Antifreeze protein, type I // Sulfotransferase domain // Excisionase-like // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCGGGCGTATATGCTGCGCGAGGGATCA |
Internal bar code: | ATTGGGTTGCAACAGGGGGCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 65 |
LEAP-Seq percent confirming: | 53.3835 |
LEAP-Seq n confirming: | 213 |
LEAP-Seq n nonconfirming: | 186 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TAGATCATCGGAATGTGCCA |
Suggested primer 2: | CTAATTCGCAGCGTTTAGGC |