| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.096779 |
| Chromosome: | chromosome 16 |
| Location: | 1347416 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651850 | (1 of 3) PTHR13367:SF8 - OTU DOMAIN-CONTAINING PROTEIN 7B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTGCATTCGCGTGCTTCAACAGTACCGC |
| Internal bar code: | CTCGTGGTGGTCCAGGCGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 41 |
| LEAP-Seq percent confirming: | 80.7692 |
| LEAP-Seq n confirming: | 231 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGCTTGGCCATTAGCTC |
| Suggested primer 2: | AGCTGGTCGCTGAGTTCAAT |