Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.096779 |
Chromosome: | chromosome_16 |
Location: | 1347663 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g651850 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCTGCAGCAGAGCGGGCAAGGGTGCGAGTC |
Internal bar code: | TGGACCAGAATTGATATCCCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 98 |
LEAP-Seq percent confirming: | 98.75 |
LEAP-Seq n confirming: | 79 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCACCATTTCTTCCTCGTCC |
Suggested primer 2: | AGCTGGTCGCTGAGTTCAAT |