| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.096782 |
| Chromosome: | chromosome 3 |
| Location: | 5531866 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186150 | (1 of 1) PF07047 - Optic atrophy 3 protein (OPA3) (OPA3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATATCCACGATGCCGTTTTACTAACCCC |
| Internal bar code: | GCCGGGGGTATATCCTTCGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1197 |
| LEAP-Seq percent confirming: | 99.7 |
| LEAP-Seq n confirming: | 8309 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGATTGTCTGCTTCACA |
| Suggested primer 2: | ACTGTCGCTTCAACATCGTG |