Insertion junction: LMJ.RY0402.096803_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.096803
Chromosome:chromosome 3
Location:4783238
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g178350 FAP272 Flagellar Associated Protein 272 similar to calmodulin; (1 of 1) PTHR23050//PTHR23050:SF190 - CALCIUM BINDING PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAACCCTGGGAGTCAATACGACCCCCAC
Internal bar code:GGGCCGGACTTATCGACTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1103
LEAP-Seq percent confirming:99.6401
LEAP-Seq n confirming:7199
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGCTCCCTATACACCCAA
Suggested primer 2:CTCAGGGAACTCCACAGCTC