| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.096815 |
| Chromosome: | chromosome 12 |
| Location: | 1941630 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g510600 | STK11,STPK11 | Serine/threonine protein kinase; (1 of 1) 2.7.11.1//2.7.11.21 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Polo kinase / Polo-like kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTCCGAGAAGTCCCTCTGAACCAGCTCC |
| Internal bar code: | AGGGTGGTTAATGTTCGCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 945 |
| LEAP-Seq percent confirming: | 99.5824 |
| LEAP-Seq n confirming: | 5246 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCAACACACCCGGTCTA |
| Suggested primer 2: | GCACTCGGTCTCACTTAGGC |