Insertion junction: LMJ.RY0402.096847_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.096847
Chromosome:chromosome 17
Location:167226
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g697334 (1 of 10) PF13519 - von Willebrand factor type A domain (VWA_2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTAATCCCAGCCCCACACCTGTGAGCCG
Internal bar code:TGAACTGCTCCATCCAGTCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:116
LEAP-Seq percent confirming:16.0226
LEAP-Seq n confirming:369
LEAP-Seq n nonconfirming:1934
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCCGCTAACTACTTCCA
Suggested primer 2:ATCCACAGCACCGGACTTAC