| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.096860 |
| Chromosome: | chromosome 12 |
| Location: | 4850444 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525000 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCACTAGAGCCACTCCTCACGACAGCCC |
| Internal bar code: | GAGGAAGCATCTGGCATGCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 318 |
| LEAP-Seq percent confirming: | 51.4995 |
| LEAP-Seq n confirming: | 1597 |
| LEAP-Seq n nonconfirming: | 1504 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATAAGGGAGCACGTGTGT |
| Suggested primer 2: | GTTCGGTTGCAGTTACGGTT |