| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.096877 |
| Chromosome: | chromosome 17 |
| Location: | 6194951 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741900 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATCAAACAGCTCTGGGGCCTGTGCATAC |
| Internal bar code: | GTACTCAGTCCGAAAAGGTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 99.7536 |
| LEAP-Seq n confirming: | 6072 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGGGATGATTGCTTGAA |
| Suggested primer 2: | CATAGCAGCGCATCAGGTTA |