| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.096921 |
| Chromosome: | chromosome 1 |
| Location: | 5077699 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035250 | (1 of 1) PTHR21549:SF0 - COILED-COIL DOMAIN-CONTAINING PROTEIN 112 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCTTCATCCTGCACAGCGCACGCCGCA |
| Internal bar code: | CTACTCCTTCGCGACGTTACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 99.3597 |
| LEAP-Seq n confirming: | 2483 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACTGCTCATACACCCGGC |
| Suggested primer 2: | TTGCCTTCTCAACAAATCCC |